|
|
|
|
|||||||
Similar Threads
|
||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Illumina PE primers | Zebrafish | Sample Prep / Library Generation | 0 | 05-11-2012 09:29 AM |
| SE adapters and PE primers | PaulineF | Illumina/Solexa | 2 | 02-09-2010 12:10 PM |
| Removing primers | Khanjan | 454 Pyrosequencing | 1 | 02-05-2010 12:09 PM |
| primers and adaptors | xgm-1999 | 454 Pyrosequencing | 2 | 09-22-2009 01:24 AM |
| Illumina Primers | Will | Illumina/Solexa | 1 | 08-10-2009 09:08 AM |
 |
|
|
Thread Tools |
01-11-2013, 09:08 AM
|
#1 |
|
Member
Location: US Join Date: Mar 2012
Posts: 36
|
V4 region primers
Hi Everyone,
I may be asking very basic question but I want to know what does 'H', 'V' and 'W' indicate in the reverse primer of V4 region of 16s rRNA. The primer sequence is GGACTACHVGGGTWTCTAAT. Thanks!!!! |
|
|
|
01-11-2013, 10:35 AM
|
#2 | |
|
Member
Location: Guilford, CT and S.F., CA Join Date: Jan 2010
Posts: 64
|
I believe those represent IUPAC nucleotide ambiguity codes:
http://www.bioinformatics.org/sms/iupac.html H = A, C, or T V = A, C, or G W = A or T Quote:
|
|
|
|
|
|
01-11-2013, 11:10 AM
|
#3 | |
|
Member
Location: US Join Date: Mar 2012
Posts: 36
|
Quote:
|
|
|
|
|
|
01-13-2013, 11:57 PM
|
#4 |
|
Member
Location: Leiden, The Netherlands Join Date: Sep 2012
Posts: 28
|
I know that wikipedia is not the most reliable site in the world, but for this it is correct. So if you ever want to know what everything is: http://en.wikipedia.org/wiki/Nucleic_acid_sequence
|
|
|
|
|
| Thread Tools | |
|
|
