Go Back   SEQanswers > Bioinformatics > Bioinformatics

Similar Threads
Thread Thread Starter Forum Replies Last Post
GFF to fasta (intergenic regions and introns) gaby Bioinformatics 25 10-27-2014 05:54 AM
"coverage" of introns, intergenic regions for RNASEQ PFS Bioinformatics 2 09-07-2011 02:51 PM
high percetage of intergenic reads from RNA-SEQ CC_seqanswers Bioinformatics 3 03-07-2011 07:48 AM
Length exon, intron, promoter and intergenic regions in human genome khb Bioinformatics 1 01-17-2011 02:03 PM
RNA-Seq: Intergenic and Repeat Transcription in Human, Chimpanzee and Macaque Brains Newsbot! Literature Watch 0 07-10-2010 03:40 AM

Thread Tools
Old 04-17-2013, 01:51 PM   #1
Senior Member
Location: Pittsburgh

Join Date: Feb 2010
Posts: 151
Default Bacteria intergenic regions RNA-seq

If I am specifically interested in differential expression of intergenic regions in bacterial RNA-seq. Do I need to have riodepleted/ ribo zero libraries or polA should work fine?

honey is offline   Reply With Quote
Old 04-18-2013, 08:07 AM   #2
Location: Canada

Join Date: Nov 2008
Posts: 37

Well, poly-A enrichment for bacterial RNA will not work because the RNA molecules are not typically poly-adenylated unless for degradation. You will have to do some kind of mRNA enrichment/rRNA depletion otherwise there will be excessive rRNA molecules sequenced and not much else.
Jean is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 03:07 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO