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Old 04-17-2013, 01:51 PM   #1
honey
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Default Bacteria intergenic regions RNA-seq

If I am specifically interested in differential expression of intergenic regions in bacterial RNA-seq. Do I need to have riodepleted/ ribo zero libraries or polA should work fine?

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Old 04-18-2013, 08:07 AM   #2
Jean
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Well, poly-A enrichment for bacterial RNA will not work because the RNA molecules are not typically poly-adenylated unless for degradation. You will have to do some kind of mRNA enrichment/rRNA depletion otherwise there will be excessive rRNA molecules sequenced and not much else.
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