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Old 07-15-2013, 05:52 AM   #1
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Location: Sweden

Join Date: Jun 2012
Posts: 5
Default DESeq-biological replicates differ between groups

I test for differential expression genes, where I have 6 biological replicates in one condition and 18 in the other.

When I use the "estimateDispersions" , it gives me the following error:

>cds <- estimateSizeFactors(countdata)
>cds <- estimateDispersions(cds)

In parametricDispersionFit(means, disps) : Dispersion fit did not converge.

Do you know why this warning arise?

Last edited by pmgr; 07-15-2013 at 06:39 AM.
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deseq, estimatedispersion

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