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Old 08-27-2013, 01:54 AM   #1
sphil
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Default ChiP-Seq experiment 101

Hey,

using ChiP-Seq one can find DNA-Binding sites etc. Therefore you have to know the protein binding to the promoter region (right?). Is there any indication of how many Proteins one can tag? So, is there a maximum of Proteins to survey at once?

Thanks,

phil
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Old 08-27-2013, 08:14 AM   #2
dpryan
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Well, typically you do ChIP-seq by performing an IP with an antibody raised against your protein of interest. So then you're just doing one protein at a time (unless your antibody targets a protein complex, in which case you're getting whatever exactly the antibody is specific for). I suppose you could also epitope tag a bunch of proteins and then use the appropriate antibody to get all of them, but I expect that'd be much more work.
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Old 08-27-2013, 02:05 PM   #3
crazyhottommy
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I do regular ChIP a lot. and usually, you can only look at one Transcription factor at once.
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Old 08-28-2013, 01:15 AM   #4
sphil
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Thank you!
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