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  • ORF Finder-Metagenome

    I've finished my first metagenome assembly and would like to know if there is a good ORF finder I could try out before proceeding to functional annotation with CAMERA for example.

  • #2
    Prodigal is really good. It's got a *meta* switch.

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    • #3
      Thank you, I also found out CAMERA has an ORF finder as well.

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      • #4
        vanillasky...are you mining assemblies for specific genes/enzymes?

        i'm trying to figure out a more automated way of picking out ORFs that relate to specific enzyme families.

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        • #5
          Hi Lac302! Maybe you can clarify what you mean by mining assemblies? I am interested in annotating the contigs coming out of my assembled metagenomes. Ultimately, yes I would like to focus on those contigs containing genes related to a certain function.

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