Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
Non-specific band after gel purification of PCR products omnivore Sample Prep / Library Generation 3 04-05-2017 07:19 PM
How to build combined library for repeatmasker? Shishir Bioinformatics 11 03-01-2015 07:41 PM
Library invisible on gel, despite Nanodrop results. Gel extraction problem? thdybwf Sample Prep / Library Generation 5 09-24-2012 05:41 AM
Can't see my library on a gel Mollycule Sample Prep / Library Generation 1 09-06-2012 09:23 PM
Library on gel but not on Bioanalyzer pauji Sample Prep / Library Generation 6 05-13-2012 06:30 PM

Thread Tools
Old 02-23-2017, 01:56 AM   #1
Junior Member
Location: Sweden

Join Date: Aug 2016
Posts: 9
Default 2500bp band on gel after mRNA library build

I'm having problems with an extra band on the gel following my library preparation (I've used Illumina TruSeq Stranded mRNA kit). At first I had two extra bands, one of smaller fragments and one larger, beside the smear from my library. The smaller one was cleared out following Illuminas instructions to do a second final cleanup. But the large band around 2500bp still remained.

The suggestion from Illumina was that it was beads that had tagged along so I was adviced to make another 5-10 min on the magnetic stand. However, the band is still there. Anyone that have had the same problem and have any suggestions how to deal with it?

The samples have been run on a RNA-tape on TapeStation, two samples, the last one in duplicate.
Attached Images
File Type: jpg Gel.jpg (10.7 KB, 21 views)
Misa is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 03:22 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO