Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • ERROR MESSAGE: Input files reads and reference have incompatible contigs

    Hello,
    I have to call variants from 454 data. Since I have no quality file, I use Bowtie2 for the alignement.

    Then to call the variants, I tried with GATK/UnifiedGenotyper:

    ##### ERROR MESSAGE: Input files reads and reference have incompatible contigs: No overlapping contigs found.
    ##### ERROR reads contigs = [chr1, chr2, chr3, chr4, chr5, ...
    ##### ERROR reference contigs = [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, ...

    The problem comes from the fact that fasta index uses 'chr1,etc.' while the reference I used was the one provided by Ensembl which uses '1,2,etc.'. Then I used the fasta coming with Bowtie. But I got another problem with the dbsnp version:

    ##### ERROR MESSAGE: Input files dbsnp and reference have incompatible contigs: No overlapping contigs found.
    ##### ERROR dbsnp contigs = [1, 2, 3, 4,etc.
    ##### ERROR reference contigs = [chr1, chr2, chr3,etc.

    The problem is that I can't find a version of dbsnp with 'chr'.
    Question:
    * is there a way to get dbsnp.vcf with 'chr'?
    * is there a way to transform the .bam file such that chromosom are noted with '1,2,etc.'?

    thank you,
    bests,
    tuka

Latest Articles

Collapse

  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin




    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    04-22-2024, 07:01 AM
  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Yesterday, 11:49 AM
0 responses
15 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-24-2024, 08:47 AM
0 responses
16 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
61 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
60 views
0 likes
Last Post seqadmin  
Working...
X