Hello! I am helping out with a microbiome project and my collaborators have had a problem that can best be explained by a sample naming error at the sequencing facility. They have agreed to resequence our samples but we have lost some faith! We would like to add an internal control to some samples to be able to distinguish them. I am thinking of adding some genomic bacterial DNA that would not be found on our environment to some, but not all, of the samples.
My question is technical. I wonder how much to add, and if adding a known amount of DNA to our environmental samples can affect the results in any way. I couldn't find any protocol for spiking environmental samples with bacteria for this purpose.
Any advice on what to use and how much to add would be much appreciated!
My question is technical. I wonder how much to add, and if adding a known amount of DNA to our environmental samples can affect the results in any way. I couldn't find any protocol for spiking environmental samples with bacteria for this purpose.
Any advice on what to use and how much to add would be much appreciated!
Comment