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Circos & RNA-Seq
Hi, my name is Luigi, I'm from University of Messina, Italy. I'm a PhD Student with the passion of Bioinformatic, and I¹m working on several RNA-Seq data. I recently discovered Circos, and I'm publishing my first paper on RNA-Seq. I would like to apply Circos plots in my work, but I didn't find any tutorial or satisfying guide to generate specific data files of RNA-Seq for Circos, and how to use it. Please, can you explain me how to create a plot from RNA-Seq data, and how to set specific data files to import? In detail, I would like to integrate, in the same plot (if possible): 1) Scatter Plot; 2) Heat Map 3) Volcano Plot. Moreover, I have an experiment with 3 replies and 4 time points: can I put all time points data to compare them, following the previous kind of graphics? Thanks very much in advance.
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM