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  • To find the difference in base pair position of two fasta file

    Hello,
    I am just beginner in Perl,
    I have Two fasta file of different length.
    I would like to align them to find difference in nucleotide position.

    Output should be like this
    Total length of fasta files
    First reference file: 1253630 base pair
    Second file: 4523366 base pair
    If match 2nd file is same as 1st reference file.
    Match position at base pair
    a-t 455222
    c-g 455665
    if not match out put should like this
    Mismatch position at base pair
    A-C 100025
    C-T 600045
    Result: should be in Output.txt

    I tried this code given below:

    use strict;
    use warnings;
    my $file1 = 'chr20.txt';
    my $file2 = 'chr21.txt';
    my $error = 'error.txt';
    open(my $in1, '<', $file1) or die "Cannot open file '$file1' for readi
    +ng: $!";
    open(my $in2, '<', $file2) or die "Cannot open file '$file2' for readi
    +ng: $!";
    open(my $out, '>', $error) or die "Cannot open file '$error' for writi
    +ng: $!";
    my $lineno = 1;
    while (my $line1 = <$in1>)
    {
    my $line2 = <$in2>;
    print "$. : $line2,$line1,";
    printf $out "Error:lineno:%d mismatch found \n", $lineno
    unless $line1 eq $line2;

    ++$lineno;
    }
    close $out or die "Cannot close file '$error': $!";
    close $in2 or die "Cannot close file '$file2': $!";
    close $in1 or die "Cannot close file '$file1': $!";

  • #2
    can anybody help me to short out the problem?

    Comment

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