Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • HELP!!!! How stable are Nextera Index primers?

    The problem has been solved, thanks a lot!
    Last edited by Phuray; 08-17-2015, 05:41 AM.

  • #2
    They are probably fine, but if you don't want to take a chance, you could phone Illumina tech support and ask them. They might be willing to replace the primers.

    --
    Phillip

    Comment


    • #3
      I would simply test a few primers with index-PCR, and run on BA side by side.

      Comment


      • #4
        they are quite robust, I have been freezing and thawing them probably 10 times or more and they were still fine. Unless you are using 50 ng of input DNA for your Nextera library prep, you have a huge excess of index primers anyway, so if part of them went bad, you are still safe.
        We use the Nextera XT kit, start from <500 pg for tagmentation and dilute the primers 1:5 to ame them last longer. And, even then, if we don´t do a bead clean up afterwards, we still see a huge peak of unused adaptors in the prep.

        Comment

        Latest Articles

        Collapse

        • seqadmin
          Strategies for Sequencing Challenging Samples
          by seqadmin


          Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
          03-22-2024, 06:39 AM
        • seqadmin
          Techniques and Challenges in Conservation Genomics
          by seqadmin



          The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

          Avian Conservation
          Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
          03-08-2024, 10:41 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by seqadmin, 03-27-2024, 06:37 PM
        0 responses
        12 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 03-27-2024, 06:07 PM
        0 responses
        11 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 03-22-2024, 10:03 AM
        0 responses
        53 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 03-21-2024, 07:32 AM
        0 responses
        68 views
        0 likes
        Last Post seqadmin  
        Working...
        X