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What could be the reason for this bioanalyzer results for a total RNA stranded lib prep? I have been using the low mammalian input kit from clontech.
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Could you post a higher-resolution version of this image and an electropherogram of the template RNA itself? This looks like it might be the result of degraded RNA. -
I see the same in my positive control which is suppose to be RNA of good quality.Comment
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They could both be degraded if you had RNase contamination at some step. How do the RNAs look on the Bioanalyzer?Comment
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What organism did the starting RNA material come from? A "spiky" RNA-seq library trace such as this often indicates the presence of high abundance transcripts. It is possible that your ribosomal depletion step was not very efficient.Comment
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