Dear all,
We just go a quotation for our Miseq runs and the cost of the TruSeq kit is way too high for regular use as we intend to use this in long term
1. Are there any published methods of in house library prepation that is Miseq compatible?
2. Are Miseq adaptors the same as Hiseq? I have found a document with Hiseq adaptor sequences but not Miseq
3. What are the cheaper alternative library preparation kits availabe?
We plan to sequence PCR amplicons than long fragments so would not be needing a shearing step.
Any suggestions are welcome or rather we are desperate for ideas!
Thanks a lot
We just go a quotation for our Miseq runs and the cost of the TruSeq kit is way too high for regular use as we intend to use this in long term
1. Are there any published methods of in house library prepation that is Miseq compatible?
2. Are Miseq adaptors the same as Hiseq? I have found a document with Hiseq adaptor sequences but not Miseq
3. What are the cheaper alternative library preparation kits availabe?
We plan to sequence PCR amplicons than long fragments so would not be needing a shearing step.
Any suggestions are welcome or rather we are desperate for ideas!
Thanks a lot
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