I have used the epicentre scriptseq kit to generate barcoded cdna libraries that I will sequence via illumina's hiseq. I am pleased with the fragmentation with this kit-- I performed 18 cycles of amplification and have an obvious product at ~300bp. I used an spri beads (agencourt ampure xp) to clean these up following the PCR, but the bioanalyzer reports that I have two very discrete bands, one peaks at roughly 600bp and the other at the expected 300bp.
I ran the product on a gel to purify the 300bp fragments. I bioanalyzed again and see the same 2 peaks.
Any ideas about what the longer band would be greatly appreciated.
-Dave
I ran the product on a gel to purify the 300bp fragments. I bioanalyzed again and see the same 2 peaks.
Any ideas about what the longer band would be greatly appreciated.
-Dave
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