Hey guys!
I was wondering how should I interpret the quality scores coming from results from 454 GS jr cDNA (not paired end) reads?
I mean, I read a lot that its prhed-like. But as the sequencing techniques from sanger and pyrosequencing are so different from each other, I wonder if its true.
Could you help me find literature about these quality scores thing and to be able to understand by analyzing my data if the quality of my reads are good or not?
Im a little confuse about that and havent got to any conclusions so far.
Thanks a lot!
I was wondering how should I interpret the quality scores coming from results from 454 GS jr cDNA (not paired end) reads?
I mean, I read a lot that its prhed-like. But as the sequencing techniques from sanger and pyrosequencing are so different from each other, I wonder if its true.
Could you help me find literature about these quality scores thing and to be able to understand by analyzing my data if the quality of my reads are good or not?
Im a little confuse about that and havent got to any conclusions so far.
Thanks a lot!
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