Hi
I am quite new to Pyrosequencing and therefore have some strange question!
I am going to analyse 60 gut samples using GS FLX + and I am thinking about using 15 MID for every 5 similar samples. I was wondering can I compare the reads of each 5 samples together after the sequencing? or Should I merge them before downstream analysis?
Appreciate your comments.
I am quite new to Pyrosequencing and therefore have some strange question!
I am going to analyse 60 gut samples using GS FLX + and I am thinking about using 15 MID for every 5 similar samples. I was wondering can I compare the reads of each 5 samples together after the sequencing? or Should I merge them before downstream analysis?
Appreciate your comments.