I need Illumina and Ion Torrent datasets for same organism and strain for my project work. Reference genome should also be available. I searched on Ion community, NCBI, DRA search but could find only 2-3 datasets which can be used in my project. I cannot use human genome datasets as it is too large. Can someone suggest me some other websites where I can find some more real datasets. Thanks.
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Look in http://www.ncbi.nlm.nih.gov/pubmed/22827831 & http://www.ncbi.nlm.nih.gov/pubmed/22522955. You should be able to find a number of E.coli K12 MG1655 datasets; that is the sample that the platforms love to use for test runs.
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
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