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Old 06-26-2016, 11:19 PM   #1
Location: China

Join Date: Oct 2010
Posts: 56
Default Why the pacbio isoseq data used for RNA analysis is much more than illumina data?

As we all know, for the human RNA-seq analysis, 4-6G clean data from illumina platform is enough. But when I use pacbio's ISO-seq pipline, the subread data is more than 10 times the amount of illumina data. So what causes the difference between the amout of data from this two platform?

Thanks a lot for any reply!
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Old 06-27-2016, 11:33 AM   #2
Location: San Francisco

Join Date: Sep 2015
Posts: 27

pacBio has less # of reads than illumina does, but each read is much longer.
And barcoding is more common on illumina platforms. Based on your number (4-6G), you are probably talking about barcoded library.
bowhan is offline   Reply With Quote

isoseq, pacbio, rna

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