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Old 09-20-2012, 12:27 PM   #1
lyz1030
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Default sequencing reads simulation

Hi,



Can anyone explain the results of wgsim of me? What does the character 'R', 'M', 'Y' stands for in the third column? What is the meaning of the second column? In addition, if I have two haplotypes of a individual (with konwn heterozygous and homozygous variants), how can simulate the sequencing reads of this individual?



Thanks




20 62504 A R +

20 64077 A R +

20 64771 A M +

20 67086 C Y +

20 67502 G C -

20 68336 T G -

20 68678 A R +

20 70639 T Y +

20 70788 T W +

20 73011 T Y +

20 75088 T W +

20 76026 T W +

20 76306 C Y +
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Old 09-26-2012, 08:53 AM   #2
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They are IUPAC codes, and most likely heterozygous variants.
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