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Old 04-16-2010, 04:12 AM   #1
santhosh003
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Location: India

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Default Assembling Sanger Reads

Hi,

I am new to genomics. I have got sanger reads. They are in .ab1, phd.1, seq,,scf format.
How can I do reference assembly of these files. Is there any software or program to convert all these files into a single fastq file? or is there any assembler to take all these files as input and do the assembly? What could be the process of doing reference assembly for these reads. Any help is appreciated. Thank you so much.
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Old 04-16-2010, 05:17 AM   #2
maubp
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For Sanger reads, you could try PHRED/PHRAD/consed
http://www.phrap.org/phredphrapconsed.html
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Old 04-17-2010, 10:51 AM   #3
sklages
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or MIRA3
http://sourceforge.net/apps/mediawiki/mira-assembler/

both run fine in our hands .. :-)

cheers,
Sven
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Old 04-21-2010, 11:54 PM   #4
skycreative
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Quote:
Originally Posted by maubp View Post
For Sanger reads, you could try PHRED/PHRAD/consed
http://www.phrap.org/phredphrapconsed.html
agree with you , it is very effective
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