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Old 04-16-2010, 01:36 PM   #1
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Location: Ontario

Join Date: Apr 2010
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Default Directional RNA sequencing protocols


Just wondering if anyone here had experience with the current directional RNA seq protocols and how well it performed.

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Old 04-19-2010, 09:42 AM   #2
Location: London

Join Date: May 2009
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Yes, lots hjave been speaking about it. Have a read on other threads but it works. Two days but you will need sRNA and mRNA kits and buy some other reagents in. Bit costly so I wuld ask illumina when the universal kit will be released...
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Old 04-20-2010, 09:25 AM   #3
Location: US

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I used the protocol and it worked fine. Comparable data to standard protocol which I did in parallel for the same samples.
Let me know if you have specific questions.

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Old 06-12-2010, 06:35 PM   #4
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Join Date: May 2010
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I'm gonna try both protocols next week and will let everyone know how it goes,
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