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Old 04-16-2010, 01:36 PM   #1
bluefin
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Default Directional RNA sequencing protocols

Hi,

Just wondering if anyone here had experience with the current directional RNA seq protocols and how well it performed.

thanks
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Old 04-19-2010, 09:42 AM   #2
Nik
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Hi,

Yes, lots hjave been speaking about it. Have a read on other threads but it works. Two days but you will need sRNA and mRNA kits and buy some other reagents in. Bit costly so I wuld ask illumina when the universal kit will be released...
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Old 04-20-2010, 09:25 AM   #3
das
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I used the protocol and it worked fine. Comparable data to standard protocol which I did in parallel for the same samples.
Let me know if you have specific questions.

DAS
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Old 06-12-2010, 06:35 PM   #4
link1
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I'm gonna try both protocols next week and will let everyone know how it goes,
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