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Old 08-10-2008, 11:59 PM   #1
NGS specialist
Location: Malaysia

Join Date: Apr 2008
Posts: 249
Default Public Helicos Data

Does anybody know where I could find some public Helicos sequence data e.g. sequences, quality files, etc.?

I expect these will be in short read format (SRF). The purpose is to modify our free aligner ( to handle this data type:

The questions are obvious:

1) Do we see similar error rates, quality dropoff towards 3' end as in Illumina sequences ?

2) Usable read length?

3) Any other 'surprises' unique to this platform?

Last edited by zee; 08-11-2008 at 12:02 AM.
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Old 08-11-2008, 01:38 AM   #2
Location: Shanghai, China

Join Date: Mar 2008
Posts: 16

maybe you can find some information on their open website:
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Old 08-11-2008, 09:14 PM   #3
NGS specialist
Location: Malaysia

Join Date: Apr 2008
Posts: 249

Yes, this is quite a useful resource, perhaps it even deserves its own thread. There are public datasets for Digital gene expression, resequencing and GSS.
The heliosphere open software release also contains programs, simulators, pipeline code, etc for working with helicos data.
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