Go Back   SEQanswers > Applications Forums > RNA Sequencing

Similar Threads
Thread Thread Starter Forum Replies Last Post
RNA-Seq: GFOLD: a generalized fold change for ranking differentially expressed genes Newsbot! Literature Watch 8 03-07-2016 11:17 AM
A fold change heatmap for RNA seq analysis using CuffDiff and cummerbund devking RNA Sequencing 16 12-23-2014 09:34 AM
fold change for rna seq data without replicates Francy87 Bioinformatics 0 11-12-2014 09:47 AM
edgeR: fold change reported by exactTest for zero values of rna-seq feralBiologist Bioinformatics 2 01-31-2014 08:35 AM
Cut-off values for fold change in RNA seq smalan RNA Sequencing 1 07-12-2012 10:10 AM

Thread Tools
Old 11-23-2016, 07:17 AM   #1
Junior Member
Location: Minnesota

Join Date: Nov 2012
Posts: 2
Default Question: Test fold change significany using duplicate reference transcriptome (RNA-S

Hi, All

I've also posted this on BioStars, sorry for the re-post (!

Is it possible (or reasonable) to compare a single RNA-Seq sample (without replicate) to a duplicated reference transcriptome?

In our experiment, we have sequenced the control treatment in duplicate, but all the other treatment only once. I was wonder if it is possible to use the duplicate controls as a reference to infer the significance (p-value) of the fold change in other samples?

It seems to me it is a simple t-test, but I'm not sure if it makes sense. Also, is it possible for us to use DESeq(2)?

shanshuiii is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 11:02 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO