Go Back   SEQanswers > General

Similar Threads
Thread Thread Starter Forum Replies Last Post
gene expression in log scale and qPCR miRNA expression data Sharmi General 1 03-24-2015 07:39 AM
Sequencing/CGH data correlation with Gene expression data (Microarrays, PCR)) yuri.tolkach Core Facilities 0 06-25-2014 02:45 AM
[NGS - analysis of gene expression data] Machine Learning + RNAseq data Chuckytah Bioinformatics 7 03-05-2012 04:16 AM

Thread Tools
Old 08-21-2017, 06:13 PM   #1
Junior Member
Location: Melbourne, Australia

Join Date: May 2017
Posts: 1
Default Gene mutations vs expression data

Hi everyone,

I have a rather silly question but I'm hoping someone will clear something up in my head.

Why do people study gene mutations in cancer patients (for example) rather than studying their RNA expression levels, since a mutation doesn't necessarily mean a cell will have a varied mRNA output and therefore a phenotypic change?
There must be some advantages to assessing variants at the DNA level rather than at the RNA level, right?

cellfree is offline   Reply With Quote
Old 08-22-2017, 02:13 AM   #2
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
Posts: 1,230

Main reasons:
1- Gene expression is not consistent and will vary by environment and other factors so an important gene can be missed because at the sampling time it was not expressed
2- Mutations in non-protein coding regions can affect gene regulation
3- A mutation in DNA can be detected by sequencing whole genome or targeted sequencing an not be missed
4- The aim normally is to correlate mutations with disease, response to drugs or condition
5- Library prep from DNA is straight and less expensive in comparison to RNA
nucacidhunter is offline   Reply With Quote
Old 08-31-2017, 02:03 AM   #3
Denise - Open Targets
Junior Member
Location: Cambridge

Join Date: Aug 2017
Posts: 1

There will be pros and cons for both (whether they are technical or biological). The good news is that you can focus on eQTLs, variants that affect the expression of one or more genes (nearby the location of the variant or not).
Denise - Open Targets is offline   Reply With Quote

dna, rna

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 05:16 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO