Hi everyone.
I think I have a problem with Whole Genome Amplification (WGA) using Phi29 polymerase. It likes ssDNA so much, that I have a great abundancy of ssDNA viruses after sequencing (454).
Can anyone advise me on this issue? Like any other polymerase for WGA that does not exclusively amplify ssDNA? Or some modification, whatever?
I think I have a problem with Whole Genome Amplification (WGA) using Phi29 polymerase. It likes ssDNA so much, that I have a great abundancy of ssDNA viruses after sequencing (454).
Can anyone advise me on this issue? Like any other polymerase for WGA that does not exclusively amplify ssDNA? Or some modification, whatever?