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Old 04-15-2010, 11:04 PM   #1
chen
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Post Mate pair, high GC

It shows that high GC samples may have a lower concentration after PCR.
Is there any notes to take when preparing the mate pair library with the HIGH GC samples, except the PCR process(increase the denature time).
Any suggestion would be help. Thanks.
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Old 05-04-2010, 08:55 PM   #2
nextgen
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Can you try betaine, DMSO to lower Tm?

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Originally Posted by chen View Post
It shows that high GC samples may have a lower concentration after PCR.
Is there any notes to take when preparing the mate pair library with the HIGH GC samples, except the PCR process(increase the denature time).
Any suggestion would be help. Thanks.
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Old 05-24-2010, 07:27 PM   #3
grace033
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change Enzyme
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Old 05-25-2010, 09:45 AM   #4
Michael.James.Clark
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I guess you're using Phusion HF for your library prep PCR normally. There is a high-GC version of Phusion you could try instead.
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