Hi all,
We would like to pileup millions of reads from a single amplicon for ultra-sensitive mutation detection.
Considering that SAMtools pileup is limited to several thousand reads at a given position I am wondering if you could suggest us any alternative approach or workaround.
Any feedback is highly appreciated!
We would like to pileup millions of reads from a single amplicon for ultra-sensitive mutation detection.
Considering that SAMtools pileup is limited to several thousand reads at a given position I am wondering if you could suggest us any alternative approach or workaround.
Any feedback is highly appreciated!
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