Hey all,
I am a complete novice with only the barest understanding of working command line interfaces (currently using resources at iPlant). I have a bunch of PacBio sequences and about 30X coverage with illumina (100 base pair end). I'd like to be able to correct the PacBio sequences with my illumina reads. Anyone care to give me a step by step? Alternatively, I'd happily give authorship rights to anyone who wants to help me with my correction when I publish this work. I am dealing with a non-model invasive weed species (leafy spurge- Euphorbia esula) - mostlytrying to assemble gene space with promoters to help leverage a bunch of transcriptomics (microarray) data we have generated over the last 5 years.
I am a complete novice with only the barest understanding of working command line interfaces (currently using resources at iPlant). I have a bunch of PacBio sequences and about 30X coverage with illumina (100 base pair end). I'd like to be able to correct the PacBio sequences with my illumina reads. Anyone care to give me a step by step? Alternatively, I'd happily give authorship rights to anyone who wants to help me with my correction when I publish this work. I am dealing with a non-model invasive weed species (leafy spurge- Euphorbia esula) - mostlytrying to assemble gene space with promoters to help leverage a bunch of transcriptomics (microarray) data we have generated over the last 5 years.
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