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A recent study published in Veterinary Clinical Pathology by Chu, C. P., & Nabity, M. B., provided a comprehensive assessment of the optimal miRNA isolation and library preparation methods for small RNA‐seq research using canine biofluids. For library prep analysis, they compared the NEXTFLEX® Small RNA-seq kit v3 and the Illumina® TruSeq® Small RNA-seq kit. The authors concluded that the NEXTFLEX® Small RNA-seq kit v3 is the optimal library preparation method for detection of potential biomarkers in canine serum and urine.
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM