Hello,
I am trying to use the samtools pileup command with the -c and -f flags to get the consensus sequnce, but am getting an error.
Command : samtools pileup -c -f RefAssemb.fasta Ref_Reads.aligned.bam
Error message: [fai_load] build FASTA index. samtools: faidx.c:171: fai_build: Assertion `fp' failed. Abort.
Has anybody else encountered the above error? And how did you resolve the same?
Thanks,
Anamika
I am trying to use the samtools pileup command with the -c and -f flags to get the consensus sequnce, but am getting an error.
Command : samtools pileup -c -f RefAssemb.fasta Ref_Reads.aligned.bam
Error message: [fai_load] build FASTA index. samtools: faidx.c:171: fai_build: Assertion `fp' failed. Abort.
Has anybody else encountered the above error? And how did you resolve the same?
Thanks,
Anamika
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