I'm sure this has been posted here before, but I can't find it!
I've got some gDNA libraries that are coming in as less than 2 nM with qPCR quantification after PCR enrichment. I know there's a way to adjust the MiSeq denaturation/dilution/loading protocol to allow for a lower starting concentration. Does anyone do this or remember the thread this info was posted in?
I've got some gDNA libraries that are coming in as less than 2 nM with qPCR quantification after PCR enrichment. I know there's a way to adjust the MiSeq denaturation/dilution/loading protocol to allow for a lower starting concentration. Does anyone do this or remember the thread this info was posted in?
Comment