Hi everyone,
We have made a cDNA library using the Roche rapid library prep kit, using 200ng starting mRNA (we have very little starting material). The fluorometer reading of the library says that the concentration is within the desired range - well above the minimum. However, when we test it on the bioanalyser to check the size range we don't get anything - just a blank electropherogram. I tested the library with a PCR for 2 genes we are interested in and they were both positive so there must be something there.
Has this happened to anyone else? Does anyone have any suggestions on how to overcome this problem?
Thanks,
Joe
We have made a cDNA library using the Roche rapid library prep kit, using 200ng starting mRNA (we have very little starting material). The fluorometer reading of the library says that the concentration is within the desired range - well above the minimum. However, when we test it on the bioanalyser to check the size range we don't get anything - just a blank electropherogram. I tested the library with a PCR for 2 genes we are interested in and they were both positive so there must be something there.
Has this happened to anyone else? Does anyone have any suggestions on how to overcome this problem?
Thanks,
Joe
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