I would like to somehow visualize phosphorothioate oligonucleotides or use another tool to ascertain whether adding S-Oligos into an active cell cultures allows for entry and possibly integration into the genome.
From this article, it appears that someone has successfully done so in bacteria, but they introduced a mutated oligonucleotide that integrated into the bacterial genome, and the mutation conferred some apparent phenotype.
Click here for article
My experiment should be innocuous in terms of phenotype, but I still need a way to know if it entered and integrated.
Thanks!
Your BRO.
From this article, it appears that someone has successfully done so in bacteria, but they introduced a mutated oligonucleotide that integrated into the bacterial genome, and the mutation conferred some apparent phenotype.
Click here for article
My experiment should be innocuous in terms of phenotype, but I still need a way to know if it entered and integrated.
Thanks!
Your BRO.
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