Normalizing microRNA seq data

[Anelda]

Hi there,

I'm trying to find out what is an acceptable way of normalizing microRNA deep sequencing data.

We have a number of samples (biological replicates). I would like to see which known micrornas are expressed in these. We are also running more experiments under various conditions and I'd like to compare these with the baseline experiments.

I have found two methods - TPM and dividing by the total number of reads in the library.

Does anyone have ideas/experience with this?

Thanks!