SEQanswers

Go Back   SEQanswers > General



Similar Threads
Thread Thread Starter Forum Replies Last Post
Truseq RNA kit for making DNA library elisadouzi Sample Prep / Library Generation 3 07-17-2013 07:32 AM
Which DNA Pol is equivalent to TruSeq kit? jlove Illumina/Solexa 4 04-29-2011 10:42 AM
Plasmid DNA Extraction Kit Orochem Vendor Forum 1 07-29-2010 09:54 AM
Successfull DNA conversion by EZ DNA Methylation-Gold kit??? nedoluzhko Sample Prep / Library Generation 1 07-21-2010 09:30 PM
Buying reagents from outside source to replace the 454 DNA library kit lmsiew 454 Pyrosequencing 2 07-07-2009 01:39 PM

Reply
 
Thread Tools
Old 04-19-2011, 04:50 PM   #1
bbsinfo
Member
 
Location: Mars

Join Date: Apr 2011
Posts: 19
Default The DNA extracted by kit contain only nucleus DNA?

Hey, I am very new, please bare with my stupid question. Does the DNA extracted by extraction kit contain only nuclear DNA? Or it also contain organalle DNA, such as mitochondrial DNA and plasmid DNA?
Thanks

Last edited by bbsinfo; 04-20-2011 at 07:16 AM.
bbsinfo is offline   Reply With Quote
Old 04-20-2011, 06:24 AM   #2
tonybolger
Senior Member
 
Location: berlin

Join Date: Feb 2010
Posts: 156
Default

Quote:
Originally Posted by bbsinfo View Post
Hey, I am very new, please bare with my stupid question. Does the DNA extracted by extraction kit contain only nucleus DNA? Or it also contain organalle DNA, such as mitochondrial DNA and plasmid DNA?
Not that i've used kits, but given the major pain that organelle DNA usually is, you're unlikely to get rid of it without major effort - selective lysing, centrifuge gradients etc.

Even then, you're talking "enriched for nuclear DNA" rather than "pure nuclear DNA".

Plant DNA extraction, depending on the species, also has to deal with a lot of secondary metabolites, which can require quite a brew of counter-measures in the buffers.
tonybolger is offline   Reply With Quote
Old 04-20-2011, 06:59 AM   #3
biznatch
Senior Member
 
Location: Canada

Join Date: Nov 2010
Posts: 124
Default

I think it depends on the kit. Unless the kit specifies nuclear DNA you're probably getting everything. I do lots of RNA extraction and that is the case. I have to add extra steps to get just nuclear RNA.
biznatch is offline   Reply With Quote
Old 04-20-2011, 06:59 AM   #4
bbsinfo
Member
 
Location: Mars

Join Date: Apr 2011
Posts: 19
Default

Quote:
Originally Posted by tonybolger View Post
you're unlikely to get rid of it without major effort - selective lysing, centrifuge gradients etc.
You have to do extra selective lysing or centrifuge before you perticipate your DNA pallets, am I right? Many thanks!
bbsinfo is offline   Reply With Quote
Old 04-20-2011, 07:03 AM   #5
bbsinfo
Member
 
Location: Mars

Join Date: Apr 2011
Posts: 19
Default

And I also have another confusion. I realized that some people believe that if you mention "genomic DNA" that only means nuclear DNA. But I have read paper, in which people use "genomic DNA" to refer both nuclear DNA, and organelle DNA.
So what is more precise? Thanks
bbsinfo is offline   Reply With Quote
Old 04-21-2011, 05:08 AM   #6
yaximik
Senior Member
 
Location: Oregon

Join Date: Apr 2011
Posts: 205
Default

The vast majority of kits designed to isolate just double stranded DNA, which means any kind of dsDNA. Because mtDNA present in larger molar amounts per cell, you get more copies of mtDNA.
yaximik is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 12:42 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO