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Old 09-16-2013, 03:02 AM   #1
Vinz
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Default HiSeq2500 Read Length

When introducing the HiSeq2500 Illumina promised 2x250bp by 2nd half of 2013. On the specification page they still list 2x150pb. Anyone has information or experience regarding longer runs in rapid run mode?
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Old 09-16-2013, 03:08 AM   #2
GenoMax
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Unless you absolutely need the throughput ...

MiSeq supports 2 x 300 bp runs with v.3 chemistry now.
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Old 09-16-2013, 03:38 AM   #3
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Indeed we would need the throughput... We have a couple of MiSeqs in operation.
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Old 09-16-2013, 03:56 AM   #4
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Among other things we are waiting for are "ordered flowcells".

(Ordered flowcells + 2 x 250 bp runs) = a winning combination

Last edited by GenoMax; 09-16-2013 at 04:00 AM.
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Old 09-16-2013, 04:21 AM   #5
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Anyone heard and theoretical numbers coming from ordered flow cells?
It would make sense for Illumina to begin roll out of new chemistries on MiSeq first due to the cost implications for any teething problems. So I expect to see them in a v4 chemisty (a part of me thought/hoped the read number jump in v3 would be down to ordered clustering).
Does anyone know if the new low diversity RTA will make it to HiSeq too?
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Old 09-16-2013, 04:57 AM   #6
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James covered the patterned flow cell in a recent blog post. He is speculating about doubling the reads and >1Tb per run on HiSeq2500.
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