Go Back   SEQanswers > Applications Forums > Metagenomics

Similar Threads
Thread Thread Starter Forum Replies Last Post
ChIP-Seq: Enabling Data Analysis on High-Throughput Data in Large Data Depository Usi Newsbot! Literature Watch 1 04-18-2018 09:50 PM
RNA-Seq, Differential Expression: a theoretical question of modeling methodology NikTuzov Bioinformatics 6 04-16-2013 08:30 AM
ChIP-Seq: Theoretical and empirical quality assessment of transcription factor-bindin Newsbot! Literature Watch 0 10-07-2010 03:11 AM
Theoretical problem for RNA-Seq Mapping RockChalkJayhawk RNA Sequencing 0 03-11-2010 07:07 AM

Thread Tools
Old 12-09-2014, 05:41 PM   #1
Junior Member
Location: Germany

Join Date: Dec 2014
Posts: 1
Default Theoretical Distribution_GC Distribution_FastQC_Metagenomic Data


I am new here and next-gen metagenomic sequencing and registered specifically to ask a question.

I recently did a sequencing project of a metagenomic source, and did an initial FastQC check.

I found the GC distribution plot interesting. I have two peaks in my sequenced data which is something that I expected and can explain. What I dont understand is how the theoretical GC distribution is determined by the software.

In the FAQ of FastQC, the following is written:

In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.

I have no idea how they get to the reference distribution.

Can anybody help?

Thanks in advance.
pumpkincactus23 is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 07:38 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO