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Old 03-30-2015, 11:54 AM   #1
Location: Florida

Join Date: Apr 2013
Posts: 13
Default Calculating concentration of whole sample based on concentration of dilution

Hello, I submitted a plate of DNA samples diluted to ~50 ng/ul (based on nanodrop values) for picogreen assay to a core lab this morning. I am trying to figure out how to figure out my actual concentration

I was wondering if I could get some help calculating my true concentration for my undiluted samples. I have attached an excel table with my original nanodrop values, my values from the diluted picogreen concentrations as well as the volumes of sample and buffer that went into each concentration.

I was wondering if someone could help me figure this out. All volumes are in microliters. Here are the first few samples. Thanks!

Sample ID Original nanodrop concentrations (undiluted) Picogreen value for diluted Volume of sample Volume of added buffer Total Volume
Y037 648.965 14.85150049 0.385 4.615 5
Y038 429.845 17.98272848 0.582 4.418 5
Y047 538.89 19.15035671 0.464 4.536 5
Y064 258.625 23.24470438 0.967 4.033 5
Y086 982.5 27.69341224 0.254 4.746 5
Y089 543.175 50.87600989 0.460 4.540 5
Y092 929.885 17.5020655 0.269 4.731 5
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