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Old 06-26-2010, 11:39 PM   #1
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Location: dhaka

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Default 3000 paired end library titration run

I have reads from 3000 Paired end library of 454. with this there is also a folder named titration run containing files of ~500MB. Can you pls tell me whether I should include this data during assembly? If yes then what are the parameters to include this? I am a real newbie in next generation sequencing. Your help will be great
sabrinaelias is offline   Reply With Quote
Old 07-01-2010, 11:29 AM   #2
Location: Oslo, Norway

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If you are sure the titration data is from the same library, and the run was successful, you definitively can include it. Titration runs are done to optimize the DNA molecule/bead ratio, and should yield good reads...
flxlex is offline   Reply With Quote

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