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Old 06-09-2020, 09:39 PM   #1
papori
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Location: berd

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Default dependent or independent samples for 16s samples of the same person at different time

Hi,
We did 2 16S microbiome sequencing experiments with 6 people in each.
The 6 people are the same 6 people in both experiments.
The only thing that have been changed is the time of the sequencing (not the time we took the sample).

Time x for experiment 1, and time y for experiment 2.

Can i treat a sequencing sample of person A in time x, and a sequencing sample of person A in time y as independent sample for statistics?

Obviously, 2 sequencing of the same person at the same time on the same run are dependant, but in case of 16s microbiome with different time and different runs?

Thanks,
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