This is very much a beginner's question, but does anybody have experience with cDNA synthesis on RNA extracted from FFPE? We would like to ligated our own adaptors to the cDNA, so we need a CDNA synthesis system that does both first and second strand synthesis, and that would work on rather poor quality material.
Which producers / kits work best in your experience?
Thank you very much for any advice.
Which producers / kits work best in your experience?
Thank you very much for any advice.