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  • summary statistics of metagenomics

    Hey All

    I want to represent my 200 metagenome assemblies and preliminary analysis in a table. I was wondering if the following data are a good representation of summary statistics for metagenomes?

    a) Average of (Number of contigs assembled per metagenome) #from SPAdes
    b) Average of (N50 of contigs per metagenome) #from QUAST
    c) Average of (% of bacterial reads per metagenome) #from MG-RAST analysis
    d) Average of (% of reads for top bacterial functions) #from MG-RAST
    e) Average of (% of genes annotated as bacteria) #from PROKKA?
    I want to do this, but how do I find if a gene predicted as bacteria is a spurious hit or not? Is there any significance test?

    What other measures can I use to show if my metagenomes are sequenced and assembled "correctly"?

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