Hi,
I am looking for a program that can de-multiplex ~1 million or more amplicon sequences based on 10bp barcodes at either end of the sequence. A set of barcodes are associated with the forward and reverse priming sequences, so these need to be taken into consideration in order to sort amplicons into the same orientation and allocate correctly.
It would also be massively beneficial if the program could quantify variants within and across samples.
Split_libraries in Qiime seems to only work on a single barcode (??)
This also goes to Fastx barcode splitter.
jMHC does exactly what I want but is only Java based and can not handle large Illumina sized data sets.
If anyone has any suggestions I would very much appreciate it.
I am looking for a program that can de-multiplex ~1 million or more amplicon sequences based on 10bp barcodes at either end of the sequence. A set of barcodes are associated with the forward and reverse priming sequences, so these need to be taken into consideration in order to sort amplicons into the same orientation and allocate correctly.
It would also be massively beneficial if the program could quantify variants within and across samples.
Split_libraries in Qiime seems to only work on a single barcode (??)
This also goes to Fastx barcode splitter.
jMHC does exactly what I want but is only Java based and can not handle large Illumina sized data sets.
If anyone has any suggestions I would very much appreciate it.
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