Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • AmpliSeq vs TruSeq

    Now that Illumina sells AmpliSeq, I am trying understanding the difference between AmpliSeq for Illumina and TruSeq Targeted panels. From what I can tell AmpliSeq can probe for more targets, and deal with lower input and lower sample quality.
    Are there any other big differences I am missing between those libraries prep methods?

    Also, what experience or opinions does anyone have when comparing these two kits?
    Last edited by seqsuave; 03-14-2019, 12:44 PM.

  • #2
    So, we had to change from TSCA to AmpliSeq. My experience about AmpliSeq right now that this is not as good as TSCA. There is not advantage regarding library preperation, it is just different. How to analyze the AmpliSeq data was difficult since I needed the Tech Support. A manual where everything is written down would be helpful.
    First of all, the in silico coverage is lower when designing a new panel with exactly the same content as for TSCA. For TSCA we had a 100% coverage and for AmpliSeq just around 98%. We would require concierge to fill this gap but this would take another three months. Secondly, the higher the number of amplicons the lower the in silico coverage. Well, this is normal but way worse than for TSCA. Finally, we ordered a panel where the in silico coverage was a 100% but one amplicon dropped out. This was not worse as for TSCA.
    I would like to knoe the experience by other users. Or is it us?

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Strategies for Sequencing Challenging Samples
      by seqadmin


      Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
      03-22-2024, 06:39 AM
    • seqadmin
      Techniques and Challenges in Conservation Genomics
      by seqadmin



      The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

      Avian Conservation
      Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
      03-08-2024, 10:41 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Yesterday, 06:37 PM
    0 responses
    8 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, Yesterday, 06:07 PM
    0 responses
    8 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-22-2024, 10:03 AM
    0 responses
    49 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-21-2024, 07:32 AM
    0 responses
    66 views
    0 likes
    Last Post seqadmin  
    Working...
    X