Illumina small RNA adapter sequence

[chengusf]

Hi,

I am asking a help for Illumina small rna sequencing data analysis. I got the small RNA sequencing data and am doing data analysis. However I have a question of small RNA adapter sequence. Here is some part of fasta file. Could you tell me which part is adapter sequence of the adapter (GCCAAT is the bar code)? I cannot find it in the website manual. Could you do me a favor? Thanks!

@HWI-H232:62:C1AV0ACXX:8:1308:20071:200816 1:N:0:GCCAAT
CCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGT
+
@@@FAADAFHHHHJIII;FFHHIJFGHIEGIGIGHJEIIGIIIIJGIIJH
@HWI-H232:62:C1AV0ACXX:8:1308:20244:200844 1:N:0:GCCAAT
GCGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT
+
?<@44@A2<2+CBFDFGB=E18?CF9:B;BFEFE?39?FE<FFFIEEEFI
@HWI-H232:62:C1AV0ACXX:8:1308:20073:200869 1:N:0:GCCAAT
CTCTCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATA
+
@@@FFFDDHHGHDICHIIIEHII:CDHGIIIIIHGGI>GDGHIECHIIIC
@HWI-H232:62:C1AV0ACXX:8:1308:20042:200910 1:N:0:GCCAAT
ACGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC
+
@C@FDFFFHHGHHJIJJGIGIGIJGHIJHHHIIJJJJJJIIJJIGIJJJJ

[kcchan]

You'll have to look at Illumina's customer sequence letter to find all of the adapter sequences. The 3' adapters will begin with the sequence TGGAATTCTCGGGTGCCAAGG and end with the barcode and P7 adapters.

[chengusf]

I checked several samples. You are right. Thank you so much!