Hey guys, I'm trying to run blastx on a transcriptome against NCBI's database so I can get an annotation. I'm using a HPC with trinotate on it, and I'm assuming that I've installed blast+ correctly.
How do I BLAST against the NCBI website's database? I'm aware that you can configure any database file for blast+ to run data against, but what if I want to run a large amount of RNA contigs (like 60,000) against NCBI's website database? I'm aware of the -remote option, but this option will default my computer to using one core to run this job. I assume that the -remote option will let my data run on NCBI's HPCs, but I don't want my job to get timed out.
Any help? How do you approach using blastx on a large dataset?
Thanks!
How do I BLAST against the NCBI website's database? I'm aware that you can configure any database file for blast+ to run data against, but what if I want to run a large amount of RNA contigs (like 60,000) against NCBI's website database? I'm aware of the -remote option, but this option will default my computer to using one core to run this job. I assume that the -remote option will let my data run on NCBI's HPCs, but I don't want my job to get timed out.
Any help? How do you approach using blastx on a large dataset?
Thanks!
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