Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • SNP detection

    Any suggest good SNP detection software for short-reads. Currently, the software I find are mostly alignment of individual tags. Any experience with software that consolidates the tags and calls SNPs?

    Does the Illumina BeadStudio or NextGenE do any of this?

  • #2
    Maq will align to your reference, and output all the differences it finds into a nice SNPs file.

    Comment


    • #3
      Can you first align with Bowtie? Then use MAQ for this?

      Comment


      • #4
        Maq does alignments itself. If Maq can be tricked into using Bowtie output, I don't know how to do it.

        Comment


        • #5
          thanks. since bowtie is so much faster than MAQ... perhaps to harness both would be an option. Hmm... more digging needed. :P

          Comment


          • #6
            PLoS ONE. 2008;3(12):e4012. Epub 2008 Dec 24.
            Comparing platforms for C. elegans mutant identification using high-throughput whole-genome sequencing.
            Shen Y, Sarin S, Liu Y, Hobert O, Pe'er I.

            Although this paper compared SOLiD and solexa platforms for variation detection, it also compared MAQ and corona_lite. MAQ mapped more reads to the refereces, but corona-lie pick up the false negative by MAQ using the same filering rules. This was caused by the way MAQ streats two-color encoding.

            Comment


            • #7
              thanks. I was aware of this paper.. but didn't know they included MAQ as well.

              Comment


              • #8
                Originally posted by doxologist View Post
                Any suggest good SNP detection software for short-reads. Currently, the software I find are mostly alignment of individual tags. Any experience with software that consolidates the tags and calls SNPs?

                Does the Illumina BeadStudio or NextGenE do any of this?

                Maybe this one could help you: MapView 3.3 - a short reads alignment viewer with genetic variation analysis It has "SNP detection".

                Comment


                • #9
                  Originally posted by doxologist View Post
                  Any suggest good SNP detection software for short-reads. Currently, the software I find are mostly alignment of individual tags. Any experience with software that consolidates the tags and calls SNPs?

                  Does the Illumina BeadStudio or NextGenE do any of this?
                  NextGENe does that .. consolidate or condense reads to make up longer fragments to be then used for alignment to reference .. a neat approach to reduce error rates I would say..
                  --
                  bioinfosm

                  Comment


                  • #10
                    MAQ's paper can be found in Genome reserach Genome Res. 2008 18: 1851-1858 originally published online August 19, 2008, doi:10.1101/gr.078212.108:
                    Mapping short DNA sequencing reads and calling variants using mapping quality scores

                    BTW, SOAP SNP detection had come out, you can try.
                    Genome Res. published online May 6, 2009, doi:10.1101/gr.088013.108:
                    SNP detection for massively parallel whole-genome resequencing

                    Comment


                    • #11
                      Bowtie Maq

                      Heng Li & Richard Durbin recently published a new mapping package that uses Burrows-Wheeler transformation, this might combine the fast mapping of Bowtie with the maq SNP-calling

                      Fast and Accurate Short Read Alignment with Burrows-Wheeler Transform

                      http://bioinformatics.oxfordjournals...bstract/btp324

                      Originally posted by doxologist View Post
                      thanks. since bowtie is so much faster than MAQ... perhaps to harness both would be an option. Hmm... more digging needed. :P

                      Comment


                      • #12
                        Talking about SNP detection, does anybody know if it is possible to use a whole genome or a single chromosome as reference in CLC bio for SNP detection? I tried but it doesnt recognize the file as a reference and it works only with genebank files that have annotation and so on... how can I do that?

                        Comment


                        • #13
                          Was looking for SNP detection software as well and ran into this post.

                          I've used SOAPsnp but just tried Mapview 3.3 and am very happy with it. It allows one to call SNPs based on adjustable quality score, coverage value and variant frequency. For a GUI it is very fast too!

                          Comment


                          • #14
                            MagicViewer will meet your need,you can try it.
                            MagicViewer is a sophisticated assembly visualization and genetic variation annotation tool for next-generation sequencing data.
                            Last edited by baby1885; 07-12-2010, 03:43 AM.

                            Comment


                            • #15
                              You can first align with bowtie (using the -S option to get a SAM output) and then use SAMtools pileup and varFilter to get Consensus or SNP calling.



                              Hope this is handy.

                              Comment

                              Latest Articles

                              Collapse

                              • seqadmin
                                Strategies for Sequencing Challenging Samples
                                by seqadmin


                                Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
                                03-22-2024, 06:39 AM
                              • seqadmin
                                Techniques and Challenges in Conservation Genomics
                                by seqadmin



                                The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

                                Avian Conservation
                                Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
                                03-08-2024, 10:41 AM

                              ad_right_rmr

                              Collapse

                              News

                              Collapse

                              Topics Statistics Last Post
                              Started by seqadmin, Yesterday, 06:37 PM
                              0 responses
                              10 views
                              0 likes
                              Last Post seqadmin  
                              Started by seqadmin, Yesterday, 06:07 PM
                              0 responses
                              9 views
                              0 likes
                              Last Post seqadmin  
                              Started by seqadmin, 03-22-2024, 10:03 AM
                              0 responses
                              50 views
                              0 likes
                              Last Post seqadmin  
                              Started by seqadmin, 03-21-2024, 07:32 AM
                              0 responses
                              67 views
                              0 likes
                              Last Post seqadmin  
                              Working...
                              X