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Old 11-10-2014, 07:59 PM   #1
Junior Member
Location: Japan

Join Date: Nov 2014
Posts: 7
Unhappy subtraction of human genome


I am using CLC bio for a metagenomic analysis.

However, the largest part of the reads is from the human genome.

Therefore, I have to exclude the reads which align to the human genome,
at the first hand (so called digital subtraction).

CLC suggested me to use the following feature:

However, it turned out that, running this feature would require 24 - 48 GB
of computer memory.

I cannot afford such a high-spec machine.

Is it possible to run this feature on a machine with only 8GB memory,
or are there any websites which could kindly carry out this digital subtraction?

doggy is offline   Reply With Quote
Old 11-11-2014, 12:50 AM   #2
Devon Ryan
Location: Freiburg, Germany

Join Date: Jul 2011
Posts: 3,480

You're paying CLC for support, you should ask them. The community will generally not provide support for commercial software packages.
dpryan is offline   Reply With Quote

digital subtraction, metagenomic analysis

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