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Old 03-22-2010, 05:45 AM   #1
ik76
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Location: London, UK

Join Date: Jan 2010
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Default 4% agarose gels?

I was just wandering whether sopmebody have used 4% agarose gels instead of 2% for preparation of genomic or mRNA Illumina libraries.
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Old 04-01-2010, 11:39 AM   #2
jgibbons1
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I've been doing my size selection step with a 3% gel...seems to be working fine. I typically run it at 80v for 2 hours for good separation.
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