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  • Running STAR on Windows WSL

    WSL can be a great option for those that need to have a Windows OS and cannot access a Linux server.
    I have tried to run STAR from Windows WSL with Ubuntu 18 installed. First, I tried the pre-compiled binaries (last release; 2.6), and the program runs, but it does not align anything. I tried with some paired-end data with default options, but after loading the genome, it finishes "successfully" in few seconds and the Aligned.out is empty. Then, I tried compiling from source. After installing dependencies, the program compiled fine although with warnings and it runs, but again it does nothing after loading the genome (but finishes without errors). The Log.out doesn´t help finding the problem:

    Code:
    Finished loading the genome: Wed Jul 11 09:49:31 2018
    
    Processing splice junctions database sjdbN=230202,   pGe.sjdbOverhang=100
    alignIntronMax=alignMatesGapMax=0, the max intron size will be approximately determined by (2^winBinNbits)*winAnchorDistNbins=589824
    Created thread # 1
    Completed: thread #0
    Completed: thread #1
    Joined thread # 1
    ALL DONE!
    Same data and genome index runs fine in a Linux server, and I have just run Hisat2 in WSL successfully with the same fastq files

    I wonder if someone managed to run STAR on WSL. I would appreciate your feedback (and Alex's one if he sees this thread)

    Thanks

  • #2
    You may want to recreate the indexes on WSL and retry. Is the memory availabe comparable on WSL and linux?

    Comment


    • #3
      Originally posted by GenoMax View Post
      You may want to recreate the indexes on WSL and retry. Is the memory availabe comparable on WSL and linux?
      Both are i5 with 16gb. I tried your suggestion, but I have just stopped index generation after 4 days running.

      I guess I will stick to Hisat2 and/or Salmon. Both run really fast in this machine using WSL.

      Comment


      • #4
        Originally posted by Jandropu View Post
        Both are i5 with 16gb. I tried your suggestion, but I have just stopped index generation after 4 days running.

        I guess I will stick to Hisat2 and/or Salmon. Both run really fast in this machine using WSL.
        I'm also working with WSL and I was able to use STAR to index and align

        sequences (Ubuntu 16.04, STAR 2.7.0e). I'm not familiar with Ubuntu

        and all my setting are from the biostar handbook. My computer has

        32GB of ram, 4cores (i7) and ssd but uses most of the resource while

        running STAR. Just wanted to reply because this was the only thread that

        I could find about using STAR in WSL when I searched about it.

        Comment


        • #5
          So, STAR works with WSL1 but not WSL2? A related discussion in biostars

          Comment

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